Cytoplasmic Transfer

Ovarian PRP Therapy

Cytoplasmic transfer treatment has helped many women around the world on pursuing their dream of motherhood and to have a child bearing their own genes.

Cytoplasmic transfer is a method of improving egg quality and its function. It is a great solution for patients over 35, with poor egg quality and for those who would like to use their own eggs for IVF procedure.

It is done via ICSI (Intracytoplasmic Sperm Injection). In cytoplasmic transfer 10-15 % of donor cytoplasma is added to the recipent’s egg together with the sperm. The sperm donor cytoplasma is carefully selected by biologists. It contains mitochondria, enzymes and other organelles. Cytoplasma does not contain any information about the donor’s phenotype; it contains only genetic information about energy production.

The added donor cytoplasma improves recipient’s cytoplasma function during fertilization, aids early development stages of an embryo and helps with its implantation. Such a procedure (a simple injection of cytoplasma) from a healthy sperm donor is able to bring back healthy mitochondrial activity and rejuvenate the patient’s oocyte.

We especially recommend cytoplasmic transfer to patients with low egg quality, low embryo grade, especially for patients over the age of 35, suffering from premature ovarian failure, with history of previous abortions or implantation failures. Cytoplasmic transfer is also helpful for patients with embryo developmental arrest, high fragmentation of embryos, idiopathic infertility and other.

 At the moment the cost of the procedure is still considerably high as it is new technology in assisted reproduction. We hope that in the future the cost may become more affordable so more fertility patients will be able to benefit from this method. Cytoplasmic transfer greatly improves egg quality in about 50% cases of low oocyte quality. Many women who have been recommended egg donation as their only chance to become mothers are now interested in trying this method as their last chance before egg donation.

Who can benefit from Cytoplasmic Transfer Treatment?

  • Females with low AMH
  • Females who produce low quality eggs
  • Older females who would like to have a child carrying their own genes.

Selection of egg donors:

In selecting egg donors, patient must send us her picture, blood group, and fill the form and state her height, weight, ethnicity, etc... 

At our clinic, we have a very wide range of donors, and multiple nationalities. Cyprus is very well-known for its universities and students come from all over the world to study & live in Cyprus. This makes us able to have donors from all over the world. Egg donation treatment in Cyprus must be anonymous according to the laws of TRNC. 

Egg donors, who are accepted at our clinic, are checked from the following:

  • Must be between 19-26 years old
  • Chromosomal Analysis Test (Karyotype)
  • Hormone blood tests & pelvic scan
  • All infectious diseases
  • A session with our part-time psychologist at the clinic to make sure the donor has no psychology or mental problems
  • No history of mental , and genetic diseases in her family

The needed Medical Tests:

Women Hormone Blood Tests:

  • FSH (follicle stimulating hormone)
  • TSH (thyroid stimulating hormone)
  • E2 
  • Prolactin

Trans-vaginal scan (Ultrasound Scan) for ovarian & uterine assessment Ultrasound Scan:

The purpose of these scans is to measure your response to medication. More precisely, the purpose of the scan is as follows:

  • Assessment of endometrial appearance and thickness
  • Assessment of uterine conditions (to make sure there are no uterine pathologies that may interfere with our treatment).
  • Assessment of the number of follicles in the ovaries.

Semen Analysis:

A semen analysis helps assess the quality of a sperm sample with respect to key parameters including sperm volume, concentration (count) per mL, motility, morphology, PH and round cell count.

Infectious Diseases Screening:

  • Anti-HIV – test for HIV/AIDS infection
  • Anti-HCV – test for Hepatitis C virus infection
  • Rubella IgG- IgM – test for German measles infection
  • CMV IgG – test for Cytomegalovirus infection
  • HbsAg – test for Hepatitis B virus infection
  • VDRL – test for Syphilis infection
  • Anti- Hbs

How to start the treatment:



Step 1

To Read everything carefully and send all of your questions to your coordinator. Please don’t hesitate to ask as many questions as you want. Please know that you have the right to schedule a call or video call at any time to ask and get to know your coordinator and doctor. We believe the relationship between the doctor & patient is very important, as she will be performing your treatment, this is why you should be relaxed and happy with your doctor. 

Step 2

Fill the application form that is sent to you by your coordinator or you can schedule a call with your coordinator and she will ask you some questions. 

Step 3

Schedule a pelvic scan date with your Gynecologist. You also have to make the required Medical Tests. 

Step 4

Send the application form, Medical tests results, and Scan to your coordinator. 

Step 5

Once you have sent your results. Within 24 hours, your coordinator will send you an assessment letter from the doctor. This assessment letter will have everything included regarding your situation, success rates, best treatment for you, and advices. Matched egg donor profiles are sent as well. 

Step 6

At this time after everything has been organized and the egg donor profiles have been chosen, you will be receiving your medical Treatment protocol and a prescription to start your treatment.

Egg donors and Patients menstruation cycle are synchronized together to have the ability to have a fresh cycle. On the day of the egg donor retrieval patient must be in Cyprus as the partner’s sperm sample shall be handed.

Initial scan

Day one of your menstrual cycle marks day one of the treatment process with medications. On day 10 you will fly to Cyprus. Your coordinator will be in touch to advise your pick-up time for the appointments in Cyprus. 
The initial scan consists of a trans-vaginal ultrasound to check the development of your follicles. (Dr Verda may prescribe additional stimulation medication at this stage if necessary). If the eggs are ready, our dedicated nurses will administer a ovulation trigger injection that evening at your hotel. The injection stimulates the final maturation of eggs in the ovaries. The eggs will be then collected 34 to 36 hours after the injection is administered.